located in the southern Brazilian region of Campo Largo-
PR, were surface-sterilized (Petrini, 1986) and aseptically
cut, then transferred to Petri dish plates containing
solid culture media. Several controls confirmed that the
sterilization procedure was effective.
The fungi Cercospora zea-maydis (MMBF 51/08) and
Bipolaris maydis (MMBF 46/08) were kindly provided
by the Biological Institute of São Paulo, taken from the
Micoteca “Mario Barreto Figueiredo”. The Fusarium verticillioides
(CMM-1060) and Colletotrichum graminicola
(CMM-1061) were deposited in the Culture Collection
of Phytopathogenic Fungi “Prof. Maria Menezes”,
with GenBank accession numbers for the 18S rRNA of
KC335155 and KC335154.