2.5. Data analysis
For plantlet regeneration, all the experiments were set up in a Randomized
Block Design (RBD). Each experiment was repeated thrice with minimum 25 replicates per treatment for leaf regeneration and
shoot bud proliferation; while 10 replicates per treatment were
used root regeneration. The data were analyzed statistically using
SPSS ver 7.5 (SPSS Inc., Chicago, USA) and the results are expressed as
a means ± SD (Duncan, 1955).
For genetic stability analysis, the fingerprints were scored considering
fragment size at a locus as bi-allelic (present = 1, absent = 0).
Reactions with each primer were repeated at least thrice and only
those fragments that were well-resolved, ranging 200–4000 base
pairs (bp) and reproduced in each instance were scored and included
in the analysis ignoring the bands intensity. RAPD and ISSR profiles
obtained for 15 in vitro derived regenerates were compared with
mother plant.