A remarkable antidiabetic activity was also observed for bile content of Labeo rohita but the mortality of animals was higher. Bile content in 100% concentration through intraperitoneal route was showed 77.82% and 78.67% reduction of diabetes after 4 h and 6 h respectively, while administration through oral route caused reduction of diabetes by 57.27% and 75.98% after 6 h and 24 h respectively. It was also noticed that all the animals were died after 6 h and 24 h in case of 100% bile content administration through intraperitoneal and oral route respectively. Intraperitoneal administration of bile content (50% concentration) showed 50.08% and 74.98% reduction in diabetes after 4 h and 6 h, while 40.83%, 61.43% and 76.89% reduction in diabetes observed after 6 h, 24 h and 48 h of oral administration of 50% bile content of Labeo rohita respectively. All the animals were died after 24 h after intraperitoneal of 50% bile of Labeo rohita, but all the animals were alive till 48 h after oral administration.