3.4 Genotoxicity of Mn on Acanthamoeba sp. assessed by Comet Assay
Alkaline Comet assay was used to determine the genotoxicity of Acanthamoeba cell after exposed to Mn for 2
hours at 30oC in IC25 value of Mn which was 12 ppm (half of IC50 value). The DNA damage was evaluated based on
the degree of the DNA damage produced by the cells which were denoted by the scoring of 0,1,2,3 and 4. The
scoring was done as shown