In this step we have added the desired microorganism into our sterilized solution. In our process the microorganism is Saccharomyces cerevisiae,commonly known as yeast. The medium of this microorganism is made at the lab of Centre for Advances Researches in Science (CARS), Gniversity of Dhaka. Yeast can be grown in either liquid medium or on the surface of a solid agar plate. Yeast cells will grow on a minimal medium containing dextrose (glucose) as a carbon source and salts that supply nitrogen, phosphorus, and trace metals. Yeast cells grow much more rapidly in the presence of rich medium that contains reagents such as yeast extract and bactopeptone.