The objective of this research was to develop an efficient protocol for shoot regeneration
from leaf segments of the Chrysanthemum cv. Vivid Scarlet by examining the effects of
plant growth regulators, dark incubation period, gelling agents, and silver nitrate. The
highest number of shoots per explant (12.3) was regenerated from leaf explants cultured
on Murashige and Skoog (MS) medium supplemented with a combination of1 mg L1 of6-
benzyladenine (BA) and 2 mg L1 of a-naphthaleneacetic acid (NAA) under light
conditions without any initial dark period. Gelrite was the most effective gelling agent
for shoot regeneration among those tested, whereas the presence of silver nitrate
distinctly inhibited shoot regeneration. Superior plant growth and rooting was observed
on a hormone-free MS medium solidified with Gelrite. Flow cytometry analysis revealed
no ploidy variation between the regenerated plants and the mother plant grown under
greenhouse conditions. The established protocol was applicable to shoot regeneration for
four out ofsix cultivars tested. This research will facilitate the genetic transformation and
micropropagation of Chrysanthemum cultivars.
2014 Published by Elsevier Masson SAS on behalf of Acade´mie des sciences.