PCR master mix (Cat. No. M7502, Promega, Madison,WI) was used according the manufacturer’s instructions.Genomic DNA (1 mL) was the template. DNA was amplified using a 35-cycle PCR (initial denaturation,95 1C for 3 min; subsequent denaturation,95 1C for 1 min; annealing temperature, 48 1C for 1 min; extension temperature was 72 1C for 1 min and final extension was 72 1C for 5 min).