The sour orange (Citrus aurantium) juice is commonly used as flavoring and acidifying agent for vegetable
salads and appetizers in Turkey. It was aimed to determine the survival and growth pattern of Salmonella
Typhimurium and Listeria monocytogenes in sour orange juice. Different concentrations of neutralized
and un-neutralized juice samples were inoculated with each of the test microorganisms (w6 log CFU/
mL) separately and then incubated at 4 C and 37 C for seven days. It was detected both of the test
microorganisms could survive and even grow in neutralized juice samples at 37 C for two days.
However, none of them could survive at the end of seventh day of incubation at 37 C. Low incubation
temperature (þ4 C) increased the survival of the tested microorganisms. Also, it was detected that
L. monocytogenes were less resistant to the variable conditions than S. Typhimurium. It was concluded
that the antimicrobial effect of sour orange juice mainly depends on the low pH value of the product.
However, incubation time and temperature are also effective on the survival of the tested pathogens.