Chlamydia trachomatis causes a wide range of diseases that have a significant impact on public health.Acute chlamydial infections can cause fragmentation of the Golgi compartment ensuring the lipid trans-portation from the host cell. However, the changes that occur in the host cell Golgi apparatus afterpersistent infections are unclear. Here, we examined Golgi-associated gene (golga5) transcription andexpression along with the structure of the Golgi apparatus in cells persistently infected with Chlamydiatrachomatis. The results showed that persistent infections caused little fragmentation of the Golgi. Theresults also revealed that Golgi fragmentation might be associated with the suppression of transcriptionof the gene golga5.