At the end of incubation, cells were harvested with 1 mm EDTA by adding 500 μl, then shaken until homogeneous and then centrifuged at 9000 rpm for 10 min.
At the endof incubation, cells were harvested with 1 mm EDTA by adding 500 μl, then shaken until homogeneous and thencentrifuged at 9000 rpm for 10 min.