.2. Biodegradation conditions
Triplicate flasks (500 mL) containing 200 mL sterile ISP-2 medium with
diuron (4 mg L1
) were inoculated with streptomycete conidial suspension
to obtain a final concentration of 1 106 conidia mL1
. Spore suspension
was not added to control flasks to assess abiotic losses. Flasks were
incubated at 25 1C on a rotary shaker for up 15 days. Residual diuron
concentrations were measured by HPLC at regular intervals (1, 5, 10 and
15 days).
2