2.3.2. The detection of glucose
After the preparation of tree-shaped strip and glucose detection solutions, we added standard solutions of glucose
to the substrate zone with 2 _L per zone and detection solutions of glucose to the detection zone with 5 _L per zone.
The left branch was blank and a series of concentrations of glucose solutions were spotted on the right six branches
(as shown in Scheme 2). The test sheet was then allowed to be dried at room temperature for 5 min.
Then the paperbased device was dipped into the distilled water.