Rat Feeding Test
To determine whether the milk obtained from cows fed hexane (toxic)
cottonseed meal was toxic when compared to the milk obtained during the
periods in which the soybean and hydraulic cottonseed meals were fed, the
milk samples were fed to an animal known to be sensitive to cottonseed
toxicity; namely, the male weanling rat. To make the test extremely critical,
the rats were fed a milk diet supplemented only with vitamins and minerals.
Since Withers et al. (I913, I9W) have shown that certain iron salts will destroy
the toxic principles in cottonseed meal, adding iron to milk might