The Wingate test (WingT)
After familiarization, a 30-s Wingate test was performed on a mechanically braked cycle ergometer (Monark, 894E, Stockholm, Sweden). All subjects performed a standardized 5-min warm-up prior to experimental data collection. After 5 min of rest, the subjects were allowed 5 s of load-less pedalling to reach maximum cadence and were instructed to maintain maximal pedal speed throughout the 30-s period once the appropriate resistance was applied. The subjects were instructed to pedal as fast as possible with an individual braking load set at 75 g · kg−1 of body mass [24]. The following variables were determined: peak power output (PPO), mean power output (MPO) and anaerobic fatigue index (FI). PPO was the highest power generated during any 5-s period of the test, whereas MPO was calculated as the average power during the entire 30-s period of the test. The FI was calculated as the percentage of power output drop throughout the test from the maximal power output. PPO and MPO were reported in absolute values (W) and relative to body mass (W · kg−1).