Results of this study illustrate the nutritional potential of the fatty acid profile of white and black Spanish mulberry fruits. The most abundant fatty acids were linoleic, palmitic, and oleic. Linoleic acid, an essential polyunsaturated fatty acid, dominated the fatty acid profile (73.9% of total fatty acids) in all the clones. In addition, GC-FID analysis revealed that the clones MA1, MA4, MN4, and MN1 contained higher contents of fatty acids, especially of linoleic acid, than the other clones. These results showed that black and white mulberries could be considered as a natural source of “essential” fatty acids, particularly linoleic acid, and support their high potential for their use in the food, cosmetic, and pharmaceutical industries.