Bacterial inoculums were prepared by growing cells in MHB (Himedia) for 24 h at 37 °C. These cell suspensions were diluted with sterile MHB to provide initial cell counts of about 10−4 CFU/ml. The filamentous fungi were grown on Sabouraud Dextrose Agar (SDA) slants at 28 °C for 10 days and the spores were collected using sterile doubled distilled water and homogenized. Yeast was grown on Sabouraud Dextrose Broth (SDB) at 28 °C for 48 h; size of inoculums was 106 CFU/ml.