PCR reactions in a 25 ìl reaction volume was
carried out to amplify the continuous gene
segment of the PRSV coat protein gene using
the coat protein gene specific primers
(Forward 5’ CGCGTTACTGAAGTGAGC 3’ and
Reverse 5’ AAGCGGTGGATGCAGGTC 3’)
designed by using the sequence information
available in the NCBI database and Fast PCR
software.