production [8], taking into consideration estimated world annual
production of bread which is about 100 million tones [9] the
amount of generated waste can reach even 10 million tones per
year worldwide. The major factor for bread waste formation is that
part of the produced product is left unsold and is returned to the
bakery due to significant level of staling and large amount of available
assortment of bakery products which are produced in excess to
fulfill the consumers demands [10]. There are limited possibilities
for reprocessing bread waste in the bakeries. Some wastes can be
processed into bread crumbs, as a replacement of part of flour in
sourdough preparation or as animal feed. However due to often
microbial spoilage its use for human and animal nutrition could
be risky for health of the consumers. These problems are the reason
why waste bread is most often left on landfills or used as a fuel for
combustion. The most promising solution for waste bread utilization
is use it as a raw material for ethanol fuel production. Earlier
studies shown that waste bread is a high-yielding material for ethanol
fermentation [11,12].