Blood collection Fasting blood samples were collected under the deep ether anaesthesia by cardiac puncture using sterile disposable syringes at baseline (pre-treatment), day 11 (during treatment) and day 21 (after treatment). The blood samples were then centrifuged at 3000
Journal of Applied Pharmaceutical Science 01 (09); 2011: 38-42 rpm for 10 minutes to separate the serum. The serum was stored at -80°C until assays were carried out. All tests were performed according to the manufacturer’s instructions.