This study concludes that clear conditions are very importantfor PDA biosensor performance, and that sensitivity and stabil-ity depend on size, zeta potential and colorimetric response. Asensitivity biosensor is important for laboratory analysis, and theextreme condition of pH, temperature and antibody concentra-tion facilitates color transition by making easy distortion of PDA-conjugated chain [11–14] however, for intelligent packagingstorage during long periods of time, the conditions should beminimum to avoid stability problems and color change withoutbacteria’s presence. Therefore, this study helped improve sensitiv-ity and stability of PDA/antibody biosensor by manipulating thesystem’s conditions thus making the results of the present researchapplicable in food and laboratory area