number of copies of a template DNA per ml of elution buffer was calculated accordingly. Standard-curves were prepared using serial dilution of PCR products from pure cultures for the different bacterial groups (Chen et al., 2015).
number of copies ofa template DNA per ml of elution buffer was calculatedaccordingly. Standard-curves were prepared usingserial dilution of PCR products from pure cultures forthe different bacterial groups (Chen et al., 2015).