Sterile water
(5 mL) containing 0.05% (v/v) Tween-20 was added to a Petri dish
culture, and the spores were gently dislodged from the surface with
a sterile glass rod. The suspensions were filtered through three
layers of cheesecloth to remove mycelial fragments, and the suspensions
were diluted to 1
×
105 conidia mL−1 with sterile water.