4. After 1 month, Inoculums stored at 4 ° C (in refrigerator) dropped to 10 ¹ ³ cfu / ml. It reduced from start 1
log cycle. In 2th – 6th month, number of microbes was reduced indefinitely, reduced by 2 log cycle per month until
the last month. Microorganisms remain 10 ³ cfu / ml at the last month.