The relationship between the flower bud morphology, bud
size, and microspore developmental stage was determined firstly.
Different sizes of flower buds were collected and measured the
lengths. Anthers were then isolated from the buds and placed
on glass slides, stained with Carbol fuchsin solution, mashed, and
observed under a microscope (Olympus BX51, Japan). Cell size and
position of the nucleus were recorded, and one hundred pollen
grains per anther were counted in randomly chosen fields of vision
using 400× magnification to determine viability. Microspores that
were not stained by fuchsin were considered non-viable.
The relationship between the flower bud morphology, budsize, and microspore developmental stage was determined firstly.Different sizes of flower buds were collected and measured thelengths. Anthers were then isolated from the buds and placedon glass slides, stained with Carbol fuchsin solution, mashed, andobserved under a microscope (Olympus BX51, Japan). Cell size andposition of the nucleus were recorded, and one hundred pollengrains per anther were counted in randomly chosen fields of visionusing 400× magnification to determine viability. Microspores thatwere not stained by fuchsin were considered non-viable.
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