Determination of the biomass density of Chlorella sp. was performed based on optical density measurement using a Dual-Beam UV–Vis Spectrophotometer (Shimadzu UV-1800, Japan).
Absorbance reading were then analyzed using a UV-Probe 2.42
software whereas the statistical analysis were performed via
Microsoft Office Excel™ and Minitab™ 16. A 95% confidence level
was selected for determination of the significance of the optimum
dosage of Chlorella sp. inoculation for phytoremediation followed
by the optimum dosage of A. niger for microalgae biomass removal.
Graphical analyses were performed using OriginLab™ Origin Pro
9.1 software for the determination of interactions between factors.
The runs showed accurate results proven with the low standard
error values in the products yield of around (±1–10%).