In addition, in order to better study the molecule mechanism of metformin, we also analyzed the gene expression profiling between normal (C57BL6) mice and diabetic-vehicle mice, and found 43 reversed genes by metformin. These reversed genes by metformin are related with diabetic melitus, such as Atf6, Anxa2, Ppp1r10, and so on (can be found in Supplementary Table S2).