Total milk fat as
gravimetrically determined did not differ between the two extraction procedures, although numerically
a slightly lower amount (2.8% less) was extracted with the new procedure (P = 0.919). In general, the new
dichloromethane extraction method was found to be much faster, less hazardous to health (shorter
exposure time) and more cost efficient (lower cost based on solvent price and amount used); overall, it is
much more suitable for gas chromatography analysis of milk fatty acids than the older, more widely used
Rose-Gottlieb extraction method.