Seeds of Amaranthus polygamous L. var. Pusa Kirti were obtained from the Indian Agricultural
Research Institute, Pusa (New Delhi). Morphologically uniform healthy seeds of Amaranthus polygamous
were soaked in water overnight. These seeds were sown in earthen pots of 20 cm diameter and 29 cm
height. The pots were filled with about 3 kg soil: manure mixture in 3:1 proportion. The pots were watered
with tap water everyday till the fourth day. At this stage, thinning of seedlings was done so as to maintain
uniform spacing amongst them. These pots were divided in four sets. One set of pots, watered with tap
water, served as control. Remaining three sets of pots were watered with 20 mM, 40 mM and 60 mM NaCl
solutions respectively (Figure 1). In order to acclimatize the plants, the concentrations of NaCl were raised
every 3-4 days in a step-wise manner. The plants were grown for forty five days. The mature leaves of forty
five day old plants were used for estimating β-carotene, (a precursor of vitamin A) thiamine (vitamin B1),
riboflavin (vitamin B6) and ascorbic acid (vitamin C) using standard protocols. β -carotene was determined
according to the protocol of AOAC (1980). Vitamin B1 (Thiamine) and B6 (Riboflavin) were estimated
according to the method of Strohecker and Henning (1966). Estimation of vitamin C was done as per the
protocol of Birch et al. (1933). F test was used to test for the statistical significance and Student’s t-test was
use to compare the treatment means
Seeds of Amaranthus polygamous L. var. Pusa Kirti were obtained from the Indian AgriculturalResearch Institute, Pusa (New Delhi). Morphologically uniform healthy seeds of Amaranthus polygamouswere soaked in water overnight. These seeds were sown in earthen pots of 20 cm diameter and 29 cmheight. The pots were filled with about 3 kg soil: manure mixture in 3:1 proportion. The pots were wateredwith tap water everyday till the fourth day. At this stage, thinning of seedlings was done so as to maintainuniform spacing amongst them. These pots were divided in four sets. One set of pots, watered with tapwater, served as control. Remaining three sets of pots were watered with 20 mM, 40 mM and 60 mM NaClsolutions respectively (Figure 1). In order to acclimatize the plants, the concentrations of NaCl were raisedevery 3-4 days in a step-wise manner. The plants were grown for forty five days. The mature leaves of fortyfive day old plants were used for estimating β-carotene, (a precursor of vitamin A) thiamine (vitamin B1),riboflavin (vitamin B6) and ascorbic acid (vitamin C) using standard protocols. β -carotene was determinedaccording to the protocol of AOAC (1980). Vitamin B1 (Thiamine) and B6 (Riboflavin) were estimatedaccording to the method of Strohecker and Henning (1966). Estimation of vitamin C was done as per theprotocol of Birch et al. (1933). F test was used to test for the statistical significance and Student’s t-test wasuse to compare the treatment means
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