Source of actinomycetes
The actinomycete strains were isolated from heavy metal polluted and non-polluted soil samples. The isolated strains were maintained in Potato Dextrose Agar slants (supplemented with cyclohexamide [25 μg/ml] and nalidixic acid [10 μg/ml] as anti fungal and antibacterial compounds respectively) at 27oC as well as subcultured to regulate its viability during the study period.