6. Mounting
- As this step, samples were twice washed with absolute ethanol to dehydrate, then centrifuged and decanted.
- The minimum amount of tertiary butyl alcohol was dropped into centrifuge tube. Then the sample was transferred to a small vial. Silicone oil is used as a mounting media. After adding of silicone oil, it is required to leave an uncovered vial to allow evaporation of tertiary butyl alcohol for 2 days in a fume cupboard. After this, samples are readily reserved for slide preparation.
- Slides can be prepared by dropping the suspension onto a slide and covering with a cover slip.