TCs and SAs were separated using a 2.050mm Xterra MS C18 column with a 2.5 mm pore size (Waters,
Millford, MA). A 20 mL injection volume and a binary gradient with a flow rate of 0.32 mL/min were used. The column temperature was kept at 15C. Mobile phase A was water with 0.1% formic acid and mobile phase B was acetonitrile with 0.1% formic acid.