QIAmpDNA Blood Mini Kit (Qiagen, MD) according to manufac-turer’s protocol. The amount of recovered DNA was measured byNanoDrop ND-1000 spectrophotometer (Thermo Scientific Mil-waukee, WI) and stored at −20◦C.tive. Positivity was also confirmed by sequencing. PCR productswere cleaned with ExoSAP-IT (Affymetrix Inc. Cleveland, OH) andsequenced and compared to the S. stercoralis repeat sequence inGenBank (AY028262).