and mounted
on Poly-L-Lysine coated microscope
slides. The mounted sections were placed
on a slide warmer at 42°C for 12 h. The
paraffin was removed from the tissue sections
by placing the microscope slides in
Histo-Clear 2 times for 3 min, then rehydrated
in 95, 85, 70, 50, and 30% ETOH 2
times in each solution for 3 min each and a
final hydration of 5 min in ddH2O. The
leaf sections were then stained with Safranin
O for 15 min, and then washed with
running dH2O until clear. The samples
were dehydrated again in a series of alcohol
and Histo-Clear baths as described
above. A cover glass was then mounted
onto the tissue sections with paramount.
The samples were examined under a microscope
and photographed.