ิbecause mutations in this region affect both
biofilm phenotype and partitioning to an oil–water interface
(10). That BslA is stable as a monomer in aqueous solution
suggeststhat the hydrophobic residues of the cap are not surface
exposed, and there must be a conformational change to expose
the hydrophobic patch, with a corresponding energy barrier to
adsorption. To investigate this experimentally, pendant drop
tensiometry with drop shape analysis was performed on BslA
solutions at concentrations between 0.01 and 0.1 mg·mL−1