Fermentation was carried out at 16e 17 C in 15 L vats. Fermentation kinetics was monitored daily by density determination and samples were taken up to quantify sugar consumption and fermentation products. Dominance of the inoculated strains was verified by restriction analysis of mitochondrial DNA (Querol, Barrio, Huerta, & Ramon, 1992) of 20 random isolates from the appropriate dilutions grown on YPD plates.