and it can also modify functional properties of microcapsules (Chen, Chen, Liu, Lin, & Chiu, 2005). Therefore, the objective of this study was to evaluate the effect of partial replacement of RSM with prebiotic agents (inulin, oligofructose, and oligofructose-enriched inulin) on the viability of Bifidobacterium BB-12, microencapsulated by spray drying, during storage for 180 days at 4 °C and at −18 °C, as well as to characterize the microcapsules in relation to their physical properties.