An alternative clean-up method using KOH and a combination
of sucrose and KCl, followed by either autoclaving or
homogenization to break the eggs, has been reported, and the
detection limit was 10 EPG in the PCR diagnosis of Clonorchis
sinensis.In our study, the lowest egg count detected was 31
EPG in the case of egg-positive samples and probably lower
than this in the case of egg-negative samples.