The test organisms were prepared in nutrient broth (Difco) and incubated at 30oC
for 24 hours. After incubation, the test pathogens were then streaked evenly onto nutrient
agar (Difco) and left for five minutes to dry before being used for the screening of
antibacterial assay. Cylindrical pieces of 1 x 1 cm size agar plugs were cut from one week
old fungi grown plates and placed on the agar previously streaked with test organisms