Sterile Erlenmeyer flasks containing 25 ml of PDB medium,150 spores and different concentrations of CS-BA nanogel, as well as free and CS-BA nanogel-encapsulated Thyme essential oils were stored in an incubator for 1 month in order to determine the MIC.Moreover, the MIC of T. vulgaris essential oils and CS-BA nanogel encapsulated T. vulgaris essential oils were studied under two
different conditions; the flasks were sealed during the experiment and other wise.
Antifungal activity (AF) was measured based on the following
equation: