In 2008, Basalma [24] conducted a study to determine the role of the combinations of TDZ and IBA for safflower cotyledonary leaves regeneration and to increase direct shoot regeneration frequency of safflower cultivar Dincer cotyledonary node and meristem tip. Cotyledonary leaves of 10 day old seedlings were cultured on MS basal medium supplemented with different concentrations and combinations of TDZ and IBA. The highest percentage of regenerated shoots (33.33%) and the highest number of shoots per explant (6.5) occurred on MS + 0.5 mg/L TDZ + 0.25 mg/L IBA. Furthermore, cotyledonary nodes and meristem tips of 14 day old seedlings were cultured on MS medium supplemented with various concentrations of 6-BA alone or combination with NAA. Direct multiple shoots from cotyledonary nodes and meristem tips developed within 18-21 days in most media tested. 100% shoot multiplication was achieved from cotyledonary node and meristem tip on a range of MS medium supplemented with different concentrations of BAP and NAA.