Aquaculture is a globally important and rapidly growing industry. It contributes positively to the
economy and sustainability of coastal communities, but it is not without regulatory challenges. These challenges are
diverse, and may include identification of fish discarded in an illegal manner, biological discharge from fish ensilage
tanks, and partially destroyed or processed tissues. Robust genetic tools are required by management authorities to
address these challenges. In this paper, we describe nine species-specific primer sets amplifying very short DNA
fragments within the mitochondrial DNA cytochrome c oxidase (COI) gene, which were designed to permit diagnostic
identification of degraded DNA from two of the most commonly farmed salmonids in Europe and North America.
Aquaculture is a globally important and rapidly growing industry. It contributes positively to the
economy and sustainability of coastal communities, but it is not without regulatory challenges. These challenges are
diverse, and may include identification of fish discarded in an illegal manner, biological discharge from fish ensilage
tanks, and partially destroyed or processed tissues. Robust genetic tools are required by management authorities to
address these challenges. In this paper, we describe nine species-specific primer sets amplifying very short DNA
fragments within the mitochondrial DNA cytochrome c oxidase (COI) gene, which were designed to permit diagnostic
identification of degraded DNA from two of the most commonly farmed salmonids in Europe and North America.
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