rapidity
of cell death for a given temperature.
At selected time points, capillary tubes were removed from the
water bath and immediately submerged in 70% ethanol on ice.
Surface-sterilized capillary tubes were blotted, transferred to milk dilution
bottles containing 20 ml peptone water, and crushed using a glass
rod. Subsequent serial dilutions were plated using acidified PDA. Plates
were incubated at 25 °C for 48 h, then enumerated.