The accuracy of the method was expressed as recovery, which is
determined by analyzing of blank samples spiked with different
concentrations of MG and LMG. The mean recoveries were in the
range of 70.2e84.8% for MG, 72.1e85.6% for LMG, and 70.3e85.7%
for MG þ LMG. The relative standard deviations were less than 9.9%
and 11.5% for repeatability and within-laboratory reproducibility,
respectively (Table 1). The results of stability test showed that the
degradation of MG and LMG in trout muscle was less than 5.5% after
8 weeks of frozen storage at e20 C (data not shown).