Cells
The human acute monocytic leukemia cell line (THP-1) was procured from American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI-1640 basal medium with supplementations of 10% fetal bovine serum (Thermo Fisher Scientific, Inc.), penicillin (100 U/cc), streptomycin (100 µg/ml) and amphotericin (0.25 µg/ml).