In this step, we need to test whether the concentration of enzyme solution meets the cleaning
requirement using Aller test kit (Fig. 3a). Firstly, sample 500 mL enzyme solution, and then get 5 mL
sample into flask using a disposable syringe. Please note that both flask and syringe need to be washed
using samples previously. Add two drops of Cl-1 reagent into flask, and the sample will change into
purple. Then, drop Cl-2 reagent into flask slowly and shake it simultaneously until the sample turned
from purple to yellow. After that, drop Cl-3 reagent into flask slowly and shake it simultaneously until the sample changed from yellow to purple, and record the consumed volume of Cl-3 reagent as V1
(Fig. 3b). The initial water was also tested according to the above process. The concentration of enzyme
solution could be calculated following the formula below
In this step, we need to test whether the concentration of enzyme solution meets the cleaning
requirement using Aller test kit (Fig. 3a). Firstly, sample 500 mL enzyme solution, and then get 5 mL
sample into flask using a disposable syringe. Please note that both flask and syringe need to be washed
using samples previously. Add two drops of Cl-1 reagent into flask, and the sample will change into
purple. Then, drop Cl-2 reagent into flask slowly and shake it simultaneously until the sample turned
from purple to yellow. After that, drop Cl-3 reagent into flask slowly and shake it simultaneously until the sample changed from yellow to purple, and record the consumed volume of Cl-3 reagent as V1
(Fig. 3b). The initial water was also tested according to the above process. The concentration of enzyme
solution could be calculated following the formula below
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