A basal practical diet (Table 1) was formulated according to the
nutritional requirements of tilapia [19] and the nutritional values of
ingredients from Brazil [20]. A commercial lyophilized probiotic
composed of B. subtilis (strain C-3102-Calsporin®, containing counts
on TSA (triptic soy agar) [22] and the values determinedwere 0.0 and
7.8 ± 0.4 106 CFUg1 of diet to control and treatment, respectively.