During the method development, limits of detection and quantification
were calculated based on threefold and tenfold signal to
noise (S/N) ratios, respectively. The LOD levels for both substrates
(pomegranate fruit and juice) were set at 0.015 lg/g (or lg/ml) for
AOH and AME, and 0.02 lg/g (or lg/ml) for TEN. The LOQ were set
at 0.05 lg/g (or lg/ml) for AOH and AME, and 0.066 lg/g (or
lg/ml) for TEN.