Field evaluation for Early blight resistance can identify the resources of resistance but the major drawbacks are the lengthy duration of the tests, uncontrolled environmental conditions necessary for infection and the presence of other pathogens (Fooled et. al., 2000, Randy et al., 2010), NC1CELBR and NC 2CELBR genotypes are determinate as a moderate resistance to early blight Alternaria solani, large-fruited, fresh-market tomato (Solanum lycopersicon L.) NCEBR-6 is advanced resistance line for early blight disease, was provided by Genetic Resource Center, University of California Davis, USA., with its origin and the crosses were obtained throughout the long term breeding program. Screening of tomato genotype against early blight occur during the different growth stages and caused a decrease in fruit quantity and quality and lead to complete defoliation.