adjusted to appropriate pH (3.5–9.0) and temperature (100–150°F) and followed by
the addition of enzyme or enzyme systems. In commercial practice the enzymes
used could be purified, semi-purified or crude from animal glands. The hydrolysis
times vary anywhere from for 1 h to more than 100 h and some manufacturers use
bacteriostatic or bactericidal preservatives especially when the hydrolysis times are
longer to prevent microbial contamination.