Bioethanol was produced from low-cost agro-industrial waste
product, apple pomace reaching maximum bioethanol concentration
using cocultures which showed better sugar and carbohydrate
consumption profile. The presence of fungi in the fermentation
flasks caused more effective sugar utilization because of their
ability to use both pentoses and hexoses. Inoculation rates of 6% (w/
v) for A.sojae and T.harzianum and 4% (w/v) for S.cerevisiae were
determined as the optimum conditions with the vented aeration
method and agitation speed of 200 rpm that gave the highest
bioethanol concentration and ethanol yield on total reducing sugar
content (YP/S) as 8.748 g/L and 0.945 g/g, respectively. To date to the
best of our knowledge, no reports are available in the literature
regarding the use of T.harzianum, A.sojae and S.cerevisiae together
for bioethanol production. Therefore, this study will serve as a base
line of the initial studies in this field. Furthermore, the results
pointed out that using cocultures can be an effective way of producing
bioethanol because of their synergistic interactions. Also
utilization of apple pomace for production of bioethanol can lead
the way of producing value added products from similar agroindustrial
wastes and provide an alternative solution to the accumulation
of lignocellulosic wastes which is a primary environmental
problem of the fruit juice industry
Bioethanol was produced from low-cost agro-industrial wasteproduct, apple pomace reaching maximum bioethanol concentrationusing cocultures which showed better sugar and carbohydrateconsumption profile. The presence of fungi in the fermentationflasks caused more effective sugar utilization because of theirability to use both pentoses and hexoses. Inoculation rates of 6% (w/v) for A.sojae and T.harzianum and 4% (w/v) for S.cerevisiae weredetermined as the optimum conditions with the vented aerationmethod and agitation speed of 200 rpm that gave the highestbioethanol concentration and ethanol yield on total reducing sugarcontent (YP/S) as 8.748 g/L and 0.945 g/g, respectively. To date to thebest of our knowledge, no reports are available in the literatureregarding the use of T.harzianum, A.sojae and S.cerevisiae togetherfor bioethanol production. Therefore, this study will serve as a baseline of the initial studies in this field. Furthermore, the resultspointed out that using cocultures can be an effective way of producingbioethanol because of their synergistic interactions. Alsoutilization of apple pomace for production of bioethanol can leadthe way of producing value added products from similar agroindustrialwastes and provide an alternative solution to the accumulationof lignocellulosic wastes which is a primary environmentalproblem of the fruit juice industry
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